diff --git a/tests/components/mipi_spi/test.esp32-ard.yaml b/tests/components/mipi_spi/test.esp32-ard.yaml
index c337c0f97d..1f0791fcc7 100644
--- a/tests/components/mipi_spi/test.esp32-ard.yaml
+++ b/tests/components/mipi_spi/test.esp32-ard.yaml
@@ -1,6 +1,7 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
+  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.esp32-c3-idf.yaml b/tests/components/mipi_spi/test.esp32-c3-idf.yaml
index 4c9cf3a946..57975e59c4 100644
--- a/tests/components/mipi_spi/test.esp32-c3-idf.yaml
+++ b/tests/components/mipi_spi/test.esp32-c3-idf.yaml
@@ -1,6 +1,7 @@
 substitutions:
   dc_pin: GPIO7
   cs_pin: GPIO8
+  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.esp32-idf.yaml b/tests/components/mipi_spi/test.esp32-idf.yaml
index ba4765ce09..f6f924c762 100644
--- a/tests/components/mipi_spi/test.esp32-idf.yaml
+++ b/tests/components/mipi_spi/test.esp32-idf.yaml
@@ -1,6 +1,7 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
+  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.rp2040-ard.yaml b/tests/components/mipi_spi/test.rp2040-ard.yaml
index a1a2a8d174..e943650475 100644
--- a/tests/components/mipi_spi/test.rp2040-ard.yaml
+++ b/tests/components/mipi_spi/test.rp2040-ard.yaml
@@ -1,6 +1,7 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
+  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20