diff --git a/esphome/components/i2s_audio/i2s_audio_media_player.cpp b/esphome/components/i2s_audio/i2s_audio_media_player.cpp
index 2b624a3917..f1f1dc0d51 100644
--- a/esphome/components/i2s_audio/i2s_audio_media_player.cpp
+++ b/esphome/components/i2s_audio/i2s_audio_media_player.cpp
@@ -109,6 +109,10 @@ void I2SAudioMediaPlayer::setup() {
     this->audio_ = make_unique<Audio>(false);
     this->audio_->setPinout(this->bclk_pin_, this->lrclk_pin_, this->dout_pin_);
     this->audio_->forceMono(this->external_dac_channels_ == 1);
+    if (this->mute_pin_ != nullptr) {
+      this->mute_pin_->setup();
+      this->mute_pin_->digital_write(false);
+    }
   }
   this->state = media_player::MEDIA_PLAYER_STATE_IDLE;
 }