diff --git a/tests/components/mipi_spi/test.esp32-ard.yaml b/tests/components/mipi_spi/test.esp32-ard.yaml
index 1f0791fcc7..c337c0f97d 100644
--- a/tests/components/mipi_spi/test.esp32-ard.yaml
+++ b/tests/components/mipi_spi/test.esp32-ard.yaml
@@ -1,7 +1,6 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
-  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.esp32-c3-idf.yaml b/tests/components/mipi_spi/test.esp32-c3-idf.yaml
index 57975e59c4..4c9cf3a946 100644
--- a/tests/components/mipi_spi/test.esp32-c3-idf.yaml
+++ b/tests/components/mipi_spi/test.esp32-c3-idf.yaml
@@ -1,7 +1,6 @@
 substitutions:
   dc_pin: GPIO7
   cs_pin: GPIO8
-  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.esp32-idf.yaml b/tests/components/mipi_spi/test.esp32-idf.yaml
index f6f924c762..ba4765ce09 100644
--- a/tests/components/mipi_spi/test.esp32-idf.yaml
+++ b/tests/components/mipi_spi/test.esp32-idf.yaml
@@ -1,7 +1,6 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
-  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20
 
diff --git a/tests/components/mipi_spi/test.rp2040-ard.yaml b/tests/components/mipi_spi/test.rp2040-ard.yaml
index e943650475..a1a2a8d174 100644
--- a/tests/components/mipi_spi/test.rp2040-ard.yaml
+++ b/tests/components/mipi_spi/test.rp2040-ard.yaml
@@ -1,7 +1,6 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
-  clk_pin: GPIO5
   enable_pin: GPIO4
   reset_pin: GPIO20