diff --git a/tests/integration/conftest.py b/tests/integration/conftest.py
index 4c798c6b72..4eb1584c27 100644
--- a/tests/integration/conftest.py
+++ b/tests/integration/conftest.py
@@ -119,6 +119,21 @@ async def yaml_config(request: pytest.FixtureRequest, unused_tcp_port: int) -> s
         # Add port configuration after api:
         content = content.replace("api:", f"api:\n  port: {unused_tcp_port}")
 
+    # Add debug build flags for integration tests to enable assertions
+    if "esphome:" in content:
+        # Check if platformio_options already exists
+        if "platformio_options:" not in content:
+            # Add platformio_options with debug flags after esphome:
+            content = content.replace(
+                "esphome:",
+                "esphome:\n"
+                "  # Enable assertions for integration tests\n"
+                "  platformio_options:\n"
+                "    build_flags:\n"
+                '      - "-DDEBUG"  # Enable assert() statements\n'
+                '      - "-g"       # Add debug symbols',
+            )
+
     return content