diff --git a/tests/components/mipi_spi/test.rp2040-ard.yaml b/tests/components/mipi_spi/test.rp2040-ard.yaml
index a1a2a8d174..85b0304cc0 100644
--- a/tests/components/mipi_spi/test.rp2040-ard.yaml
+++ b/tests/components/mipi_spi/test.rp2040-ard.yaml
@@ -1,7 +1,7 @@
 substitutions:
   dc_pin: GPIO14
   cs_pin: GPIO13
-  enable_pin: GPIO4
+  enable_pin: GPIO16
   reset_pin: GPIO20
 
 packages: